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Cas13-induced cell dormancy prevents the rise of CRISPR-resistant bacteriophage


Mojica, F.J., C.Díez-Villaseñor, García-Martínez, J. & Soria, E. The intermediate sequences of recurrently spaced prokaryotic repeats are derived from overseas genetic parts. J. Mol. Evol. 60, 174-182 (2005).


Bolotin, A., Quinquis B., Sorokin, A. and Ehrlich, S. D. Frequently spaced, recurrently spaced quick palindrome (CRISPR) repeats have extrachromosomal spacers. Microbiology 151, 2551-2561 (2005).


Pourcel, C., Salvignol, G. and Vergnaud, G. The CRISPR parts of Yersinia pestis purchase new repeats by preferential uptake of bacteriophage DNA and supply extra instruments for evolution research. Microbiology 151, 653-663 (2005).


Barrangou, R. et al. CRISPR gives acquired resistance in opposition to viruses in prokaryotes. Science 315, 1709-1712 (2007).


Brouns, S.J. et al. Small CRISPR RNAs information antiviral protection in prokaryotes. Science 321, 960-964 (2008).


Marraffini, L. A. and Sontheimer, E. J. CRISPR intrude restrict horizontal gene switch in staphylococci by concentrating on DNA. Science 322, 1843-1845 (2008).


Garneau, J.E. et al. The bacterial immune system CRISPR / Case cuts DNA from bacteriophage and plasmid DNA. Nature 468, 67-71 (2010).


Abudayyeh, O. O. et al. C2c2 is a CRISPR effector concentrating on RNA focused RNA. Science 353, aaf5573 (2016).


Smargon, A.A. et al. Cas13b is an RNase guided by RNA related to CRISPR kind VI-B differentially regulated by accent proteins Csx27 and Csx28. Mol. Cell 65, 618-630.e617 (2017).


Yan, W. X. et al. Cas13d is a kind VI compact CRISPR effector concentrating on RNA modulated positively by an adjunct protein containing a WYL area. Mol. Cell 70, 327-3393225 (2018).


Deveau, H. et al. Phage response to resistance encoded by CRISPR in Streptococcus thermophilus. J. Bacteriol. 190, 1390-1400 (2008).


van Houte, S. et al. The advantages of the variety of a prokaryotic adaptive immune system. Nature 532, 385-388 (2016).


Pyenson, N. C., Gayvert Okay., Varble A., Elemento, O. and Marraffini, L. A. Excessive specificity of concentrating on throughout immunity CRISPR – Sort III bacterial case restricts viral leakage. Cell Host Microbe 22, 343-353.e343 (2017).


Meeske, A. J. and Marraffini, L. A. The complementarity of the RNA guides prevents self-targeting in Sort VI CRISPR methods. Mol. Cell 71, 791-801e793 (2018).


Westra, E.R. et al. CRISPR immunity is predicated on the following binding and degradation of the invading DNA super-wrapped by Cascade and Cas3. Mol. Cell 46, 595-605 (2012).


Koonin, E. V. & Zhang, F. Immunity of coupling and programmed cell suicide in prokaryotes: selection of life and dying. BioEssays 39, 1-9 (2017).


Parreira, R., Ehrlich, S.D. and Chopin, M.C. Dramatic decomposition of phage transcripts in lactococcal cells bearing the determinant of abortive an infection, AbiB. Mol. Microbiol. 19, 221-230 (1996).


Fineran, P.C. et al. The abortive phage an infection system, ToxIN, capabilities as a protein-RNA toxin-antitoxin couple. Proc. Natl Acad. Sci. USA 106, 894-899 (2009).


Brief, F. L. et al. Selectivity and self-assembly within the management of a bacterial toxin by a non-coding antitoxic RNA pseudoknot. Proc. Natl Acad. Sci. USA 110, E241 to E249 (2013).


Shiloh, M. U., Ruan, J. and Nathan, C. Analysis of bacterial survival and phagocyte perform with a fluorescence-based microplate assay. Infect. Immun. 65, 3193-3198 (1997).


Watson, B.N., Staals, R.H.J. and Fineran, P.C. CRISPR-Cas-mediated phage resistance enhances horizontal gene switch by transduction. MBio 9, e02406-17 (2018).


Payne, P., Geyrhofer, L., Barton, N.H. and Bollback, J.P. CRISPR-based group immunity can restrict phage outbreaks in bacterial populations. eLife 7, e32035 (2018).


Klumpp, J. et al. Listeria A511 bacteriophage genome, non-permeable, terminally terminated: myovirus mannequin of gram-positive micro organism resembling SPO1. J. Bacteriol. 190, 5753-5765 (2008).


Rostøl, J. T. and Marraffini, L. (Ph) Phages illumination: how micro organism resist their parasites. Cell Microbe Host 25, 184-194 (2019).


Yan, W. X. et al. CRISPR methods – Circumstances of V sorts which can be functionally completely different. Science 363, 88-91 (2019).


Zetsche, B. et al. Cpf1 is a novel RNA-guided endonuclease of a CRISPR system – class 2 cell. Cell 163, 759-771 (2015).


Hynes, A. P., Villion, M. and Sparrow, S. The variation of bacterial immunity CRISPR – Case could also be brought on by faulty phages. Nat. Frequent. 4399 (2014).


Lauer, P., Chow, M.Y., Loessner, M.J., Portnoy, D.A. and Calendar, R. Building, characterization and use of two phage integration vectors particular for a Listeria monocytogenes web site. J. Bacteriol. 184, 4177-4196 (2002).


Rocourt, J., Schrettenbrunner, A., Hof, H. & Espaze, E. P. [New species of the genus Listeria: Listeria seeligeri]. Pathol. Biol. (Paris) 35, 1075-1080 (1987).


Lemaitre, JP, Duroux, A., Pimpie, R., Duez, JM and Milat, ML Listeria, induction of phage and phage tails triggered by elements of the bacterial development medium (phosphate, LiCl, nalidixic acid and acriflavine ). Appl. About. Microbiol. 81, 2117-2124 (2015).


Loessner, M.J., Inman, R.B., Lauer, P. & Calendar, R. Full sequence of nucleotides, molecular evaluation and genome construction of bacteriophage A118 of Listeria monocytogenes: implications for phage evolution. Mol. Microbiol. 35, 324-340 (2000).

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